Central Veterinary Laboratory (CVL) focuses program with the objective of securing healthy national flocks of animals and birds throughout the nation by mitigating the occurrence of diseases of livestock and poultry. CVL also works on epidemic investigation as well as surveillance and investigation on various diseases in its approved annual program. The direct benefit of the performance of various laboratories has been experienced in the field of veterinary medical care based on valid laboratory test results. To achieve these multidimensional activities, CVL works with a series of laboratory test procedures through its various laboratory sections; Pathology, Parasitology, Microbiology, Serology, Laboratory management and teaching lab and Molecular Biology with a considerable progress in the later. At present the molecular based diagnosis of avian influenza is in routine use.

CVL has standard Operating Procedures, test protocols and quality guideline manual. CVL is gradually practicing the biosafety/ biosecurity measures so that good laboratory practice is followed in our all the diagnostic laboratories. CVL has already been adopting test verification system through international reference laboratories which will help in the accreditation of CVL for international certification as well.

To provide diagnostic facilities throughout the country, CVL works through its five Regional Veterinary Laboratories (RVLs) located one in each of the development regions of the nation; eastern (Biratnagar), central (Janakpur), western (Pokhara), mid-western (Surkhet) and far-western (Dhangadhi) as well as through National Avian Disease Investigation Laboratory (NADIL) located in Chitwan. To provide the diagnostic services smoothly throughout the nation, 15 basic laboratories have been established in 15 District Livestock Service Offices (DLSOs) namely, Illam, Jhapa, Saptari, Sarlahi, Rautahat, Parsa, Makawanpur, Kabhrepalanchowk, Chitwan, Rupandehi, Dang, Banke, Jumla, Dadeldhura and Kanchanpur, and 60 primary laboratories one in each DLSOs of other districts. The basic laboratories are capable to perform parasitological examinations, microbial culture and antibiotic sensitivity test. Specimens that cannot be processed in the aforementioned laboratories due to insufficient facilities and expertise are referred to the CVL. In this way, CVL works as a reference veterinary laboratory in Nepal.